Influence of biphasic tissue culture
medium in the bud multiplication
and on the shoot elongation in vitro
of Ananas lucidus Miller.
The study was conducted at Biotechnology Laboratory
of Embrapa Tropical Agroindustry in Fortaleza
(Ceará), Brazil. The objective of this workwas to
evaluate influence of biphasic tissue culture medium
in the bud multiplication and on the shoot elongationin vitro of ornamental pineapple (Ananas lucidus
Miller). It was used bud multiplication explants ofin vitro culture with 0,5 cm of height. The tissue culture medium used was MS, solid and liquid, supplemented
with BAP (1 mg.L-1) and ANA (0,1 mg.L-1).
The statistic delineation was completely randomized
design with the following treatments: additions of
10 mL of liquid tissue culture medium per glass in
periods of cultive: at 0 day, 15 days, 30 days, 15 and
30 days, 30 and 60 days, and in each 15 days for 75
days, with 14 replicated and 6 explants per plot. The
evaluated parameters were: fresh weight matter and
dry weight matter at 90 days of culture. In this same
period the cultures of each treatments were removed
for a basic tissue culture medium MS solidified with
agar 0,4% (p/v) and supplemented with ANA (0,01
mg.L-1) for shoot elongation, using as explants clusters
of buds with 0,5 cm of diameter. The statistic
delineation was completely randomized design with
6 replicated and 6 explants per plot. There were three
successive subcultives for this same new medium in
each 30 days. At 90 days of culture in the shoots elongation
medium were evaluated the number of shoot/
explant, shoot number percentage and percentage of
shoot rooting in four amplify of height. The highest
value for fresh weight matter (913,7 mg/explant) and
dry weight matter (69,1 mg/explant) were obtained
with addition of 10 mL in the liquid tissue culture
medium at 30 days of culture. The additions of
liquid tissue culture medium at 15 and 30 days of
culture provided the highest mean of number of shoot
(32.6 shoot/explant) at 90 days of culture in the shoot
elongation medium. In all treatments the highest percentage
of number of shoot and percentage of shoot
rooting were observed in amplify of 1,0 cm to 2,0
cm of height.