Micropropagation of Syngonium podophyllum
Farmers who search for large scale and quality
seedlings, have difficulty in propagating Syngonium
podophyllum Schott (Araceae) at field due to plant
health problems. Therefore, lateral buds of S.
podophyllum leaf axils were used to micropropagate
this ornamental species. At establishment stage, they
were submitted to two disinfection procedures and
further by inoculated in MS medium with and/or
without the addition of 3-indolacetic acid (IAA) and
6-benzilaminopurine (BAP) growth regulators. At
development stage, the explants were submitted to
different BAP concentrations added to MS medium.
The buds obtained were transferred to flasks
containing MS for plant development. The
exposition of lateral axilary buds to a primary asepsis
in laboratory followed by a secondary asepsis in
laminar flow bench, proved to be more efficient in
disinfecting the explants, with 100% survival rate.
The addition of growth regulators, IAA and BAP both
at 1.0 mg.L-1, in MS medium, has promoted greater
seedling growth during in vitro plant development.
The use of high BAP concentrations in the
proliferation mean did not induce a significant
increase in average bud number per explant; however,
it resulted in significant decrease in the average
number of leaves and roots. When explants originated
from the proliferation treatment with 1.0 mg.L-1 of
BAP were cultivated in MS with no growth regulator,
greater seedling development was observed.