The present research aimed to develop a reliable technique for in vitro conservation of Aechmea bambusoides germplasm. A. bambusoides germplasm was successfully established in vitro through seeds obtained from field collection. The plant multiplication was achieved in a liquid Murashige and Skoog medium without growth regulators and its long term culture maintained at least for 1.5 year without losing their growth ability. Axillary shoots obtained from A. bambusoides in vitro grown plants were readily acclimatized in a substrate containing either Dicksonia sellowiana fiber (xaxim) or Eichhornia crassipes fiber (aguapé).