Rooting induction and acclimatization are
the most important phases during in vitro plant
propagation and may affect the process because of
its inefficiency and lack of appropriate protocols.
Although some methodologies have already been
described for in vitro propagation of bromeliads, no
protocol has yet been defined for the imperial bromeliad
(Alcantarea imperialis), an endangered native Brazilian
bromeliaceae. The objective of this work was to
evaluate the effect of MS medium supplemented with
different NAA concentrations (0.0; 0.1; 0.5; 1.0 and
2.0 mg L-1) on rooting induction of shoots obtained
through in vitro regeneration. The acclimatization of
rooted and non-rooted plantlets was also evaluated.
Although root induction was observed in all shoots in
the presence of NAA, the use of 0.1 mg L-1 is suggested
to reduce costs and the number of secondary roots
formed. Higher acclimatization efficiency is obtained
using rooted plantlets.