The IAC Botanical Garden has as objective to conserve native species of the State of São Paulo, and it has preservation areas for in situ, ex situ and in vitro, beyond chambers with low temperature once the crioconservation has become an interesting alternative for the conservation of genetic resources. The Amaryllidaceae family presents plants of high ornamental value and great commercial appeal and, for these reasons, it has been object of attention for the conservation of its natural diversity. An experiment with 3 species of recalcitrant seeds of Amaryllidaceae was carried through: Hippeastrum papilio (HP), Hippesatrum glaucescens (HG) and the Zephyrantes robusta (ZR), being 4 treatments with four repetitions of 25 seeds each. The treatments were: T1. -196OC without protector; T2. -196OC with the protector PVS2. T3. -196OC with the protector agent sodium alginate; T4. seeds sown directly with no cold treatment (control). The obtained data are: T1 - ZR, HP and HG showed 98%, 70% and 80% of germination, respectively. T2 - 93%, 68% and 21%, respectively. T3 - 1%, 0% and 8%, respectively. Finally, T4 - 99%, 72% and 88%, respectively. The results prove that the protective agents are not necessary for these species, once germination rates were better without PVS2 or sodium alginate. It was also observed that the crioconservation, without protector agent, showed the seed germination earlier than the control, then it is possible to imagine that a break of seed dormancy occurred. This concept must be confirmed.