The production of ornamental plants has gained growing importance in Brazil. Intending to supply the increasingly exigent internal and external markets led to a gradual boost in this activity. Problems related to cultivation and management of the recently introduced species as well as their sanitation may pose setbacks to Brazilian Floriculture. Aiming to contribute with strategic information concerning ornamental sanitation in Brazil, 1,439 samples were analyzed for virus presence between 2004 and 2008. Among positive samples, 117 were infected with flexuous elongated virus particles, 75 had rigid elongated particles and 10 showed isometric particles. The orchids comprised 44.2% of the total analyzed samples and only 15% of them were infected. It is worth mentioning the occurrence of an exotic virus species in Brazil, the Alternanthera mosaic virus.