Micropropagação de Trichopilia suavis Lindl. & Paxton
DOI:
https://doi.org/10.1590/2447-536X.v29i3.2653Palavras-chave:
Orquídea, protocormo, regulador de crescimento, aditivo orgânico, in vitroResumo
Trichopilia suavis Lindl. & Paxton é um dos representantes mais importante do gênero Trichopilia, que pode ser usado como vaso de plantas para decoração de interiores. além disso, suas belas inflorescências são um elemento incomum adicionado às composições de buquês e usado ativamente na produção de perfumes. Este estudo foi realizado com o objetivo de desenvolver e aprimorar o método de micropropagação para produção clonal massal de T. suavis através do cultivo de protocormos. O meio de cultura de MS (½) foi modificado pela adição de diferentes componentes orgânicos e reguladores de crescimento. Os protocormos obtidos de sementes germinadas por método assimbiótico foram utilizados como explantes. Os resultados mostraram que no tratamento com ½ MS com 0,5 mg L-1 de 6-Benzilaminopurina (6-BAP) e 100 mL L-1 de água de coco foi considerado ótimo para formação de novos protocormos (7,50 ± 0,7 unidades por protocormo). Na fase de enraizamento, o maior número e comprimento de raízes (7,0 ± 0,45 unidades por plântula, 2,64 ± 0,18 cm) foram obtidos através o cultivo subsequente de plântulas em meio ½ MS com 0,5 mg L-1 de ácido indolil-3-butírico (IBA), 1,0 g L-1 de carvão com adição de 50 g L-1 de purê de banana. As mudas obtidas foram aclimatadas com sucesso em um substrato composto por casca, perlita e turfa na proporção de 1:1:1.
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