Conservation and propagation of Drosera schwackei: cryopreservation, in vitro germination, and development of an ornamental carnivorous species
DOI:
https://doi.org/10.1590/2447-536X.v31.e312831Keywords:
conservation, Droseraceae, micropropagation, ornamental plantsAbstract
Drosera schwackei (Droseraceae) is a carnivorous species with high ornamental potential, but no established protocols exist for its cultivation and conservation. This study aimed to develop a protocol for in vitro germination, plant development, and cryopreservation of D. schwackei seeds. The experiment was conducted in a completely randomized design with a 2 x 4 factorial scheme (two concentrations of sodium hypochlorite and four immersion times), with four replications, each containing 30 seeds. The seeds were cultivated in different concentrations of MS medium (MS1/3, MS1/2, MS100%, and control with agar/water). Two experiments were carried out to evaluate the in vitro development of seedlings: 1) Testing different salt concentrations of MS medium (1/3, 1/2, and 100%); 2) MS1/3 medium supplemented with different concentrations of BAP (6-benzylaminopurine). For cryopreservation, seeds were stored in cryotubes and immersed in liquid nitrogen for 1, 6, 12, 24, 48, and 120 hours, with a control group inoculated on the same day. Disinfestation with 2% sodium hypochlorite for 10 minutes effectively stimulated germination. MS1/3 and MS1/2 media favored seed germination and seedling growth, while low concentrations of BAP promoted better plant development. The seeds survived cryopreservation for up to 120 hours in liquid nitrogen without impairing plant development or morphology. Sphagnum moss proved an efficient substrate for acclimating D. schwackei seedlings from in vitro cultivation.
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AHMED, N.; SHAHID, M.; SIOW, K.S.; WEE, M.F. M. R.; HARON, F.F.; PATRA, A.; FAZRY, S. Germination and growth improvement of papaya utilizing oxygen (O2) plasma treatment. Journal of Physics D: Applied Physics, v.55, n.25, 255205, 2022. https://doi.org/10.1088/1361-6463/ac6068
BERTSOUKLIS, K.; THEODOROU, P.; ARETAKI, P.E. In vitro propagation of the mount parnitha endangered species Sideritis raeseri subsp. Attica. Horticulturae, v.8, n.12, p.1114, 2022. https://doi.org/10.3390/horticulturae8121114
BRAZIL. Ministério da Agricultura, Pecuária e Abastecimento. Regras para análise de sementes. Brasília: Ministério da Agricultura, Pecuária e Abastecimento, 2009. p.395.
CROSS, A.T.; KRUEGER, T.A.; GONELLA, P.M.; ROBINSON, A.S.; FLEISCHMANN, A.S. Conservation of carnivorous plants in the age of extinction. Global Ecology and Conservation, v.24, e01272, 2020. https://doi.org/10.1016/j.gecco.2020.e01272
DARWIN, C. R. Insectivorous plants. D. New York: Appleton & Co., 1875. 462p.
DEINLEIN, U.; STEPHAN, A.B.; HORIE, T.; LUO, W.; XU, G.; SCHROEDER, J.I. Plant salt-tolerance mechanisms. Trends in Plant Science, v.19, n.6, p.371-379, 2014. https://doi.org/10.1016/j.tplants.2014.02.001
ELLISON, A.M.; GOTELLI, N.J. Energetics and the evolution of carnivorous plants – Darwin’s ‘most wonderful plants in the world’. Journal of Experimental Biology, v.60, n.1, p.19–42, 2009. https://doi.org/10.1093/jxb/ern179
FLEISCHMANN, A.; CROSS, A.T.; GIBSON, R.; GONELLA, P.M.; DIXON, K.W. Systematics and Evolution of Droseraceae. In: ELLISON, A.; ADAMEC, L. (Eds.). Carnivorous plants: physiology, ecology and evolution. Oxford: University Press, 2018. p.45-57. http://doi.org/10.1093/oso/9780198779841.001.0001
GONELLA, P.M.; RIVADAVIA, F.; FLEISCHMANN, A. Drosera magnifica (Droseraceae): the largest New World sundew, discovered on Facebook. Phytotaxa, v. 220, n.3, p.257–267, 2015. http://dx.doi.org/10.11646/phytotaxa.220.3.4
GONELLA, P.M.; SANO, P.T.; RIVADAVIA F.; FLEISCHMANN, A. A synopsis of the genus Drosera (Droseraceae) in Brazil. Phytotaxa, v.553, p.1-76, 2022. https://doi.org/10.11646/phytotaxa.553.1.1
JAN, S.; SINGH, R.; BHARDWAJ, R.; AHMAD, P.; KAPOOR, D. Plant growth regulators: a sustainable approach to combat pesticide toxicity. 3 Biotech, v.10, n.466, p.1-12, 2020. https://doi.org/10.1007/s13205-020-02454-4
JAYARAM, K.; PRASAD, M.N.V. Rapid in vitro multiplication of Drosera indica L.: a vulnerable, medicinally important insectivorous plant. Plant Biotechnology Reports, v.1, p.79–84, 2007. https://doi.org/10.1007/s11816-007-0014-7
JOSE, J.V. Physiological and molecular aspects of macronutrient uptake by higher plants. In: Sustainable Plant Nutrition: Molecular Interventions and Advancements for Crop Improvement; Cambridge: Academic Press, 2023. p.1-21. https://doi.org/10.1016/B978-0-443-18675-2.00010-9
JUNIPER, B.E.; ROBINS, R.J.; JOEL, D.M. The carnivorous plants. London: Academic Press Limited, 1989. 353pp.
KASHYAP, P.; KUMAR, S.; SINGH, D. Performance of antifreeze protein HrCHI4 from Hippophae rhamnoides in improving the structure and freshness of green beans upon cryopreservation. Food Chemistry, v.320, 126599, 2020. https://doi.org/10.1016/j.foodchem.2020.126599
KATOGI, T.; HOMAN, Y.; TAKAHASHI, C.; SHIRAKAWA, J.; HOSHI, Y. A Comparative study of biseriate glandular trichomes of allopolyploid species Drosera tokaiensis and Its parental species. Cytologia, v.87, n.4, p.353-361, 2022. https://doi.org/10.1508/cytologia.87.353
KUMAR, S.; MISHRA, S. 2021. Sundews. Available at: https://www.researchgate.net/publication/352172450. Accessed on: jun. 09 2024.
MAGUIRE J.D. Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science, v.2, p.176-177, 1962.
MEHBUB, H.; AKTER, A.; AKTER, M.A.; MANDAL, M.S.H.; HOQUE, M.A.; TULEJA, M.; MEHRAJ, H. Tissue culture in ornamentals: cultivation factors, propagation techniques, and its application. Plants, v.11, p.,3208, 2022. https://doi.org/10.3390/plants11233208
MIHOVILOVIĆ, B.A.; KEREŠA, S.; LAZAREVIĆ, B.; TOPOLOVEC PINTARIĆ, S.; MARTINKO, K.; MARKOVIĆ, Z.; TURKALJ, K.; HABUŠ JERČIĆ, I. The use of sodium hypochlorite and plant preservative mixture significantly reduces seed-borne pathogen contamination when establishing in vitro cultures of wheat (Triticum aestivum L.) Seeds. Agriculture, v.14, n.4, p.556, 2024. https://doi.org/10.3390/agriculture14040556
MOSA, K.A.; AHMED, A.E.; HAZEM, Y.; KANAWATI, I.S.; ABDULLAH, A.; HERNANDEZ-SORI, L.; ALI, M.A.; VENDRAME, W. Insights into cryopreservation, recovery and genetic stability of medicinal plant tissues. Fitoterapia, v.169, 2023. https://doi.org/10.1016/j.fitote.2023.105555
NAZIR, U.; GUL, Z.; SHAH, G.; KHAN, N. Interaction effect of auxin and cytokinin on in vitro shoot regeneration and rooting of endangered medicinal plant Valeriana jatamansi Jones through tissue culture. American Journal of Plant Sciences, v.13, p.223-240, 2022. https://doi.org/10.4236/ajps.2022.132014
PÊGO, R.G.; PAIVA, P.D.O.; PAIVA, R. Micropropagation of Syngonanthus eleganthulus. Ciência e Agrotecnologia, v.37, p.32-39, 2013.
POPOVA, E.; KULICHENKO, I.; KIM, H.H. Critical role of regrowth conditions in post-cryopreservation of in vitro plant germplasm. Biology, v.12, p.542, 2023. https://doi.org/10.3390/biology12040542
PRADA, J.; AGUILAR, M.; ABDELNOUR-ESQUIVEL, A.; ENGELMANN, F. Cryopreservation of Seeds and Embryos of Jatropha curcas L. American Journal of Plant Sciences, v.6, p.172-180, 2015.
REIS, E.S.; PINTO, J.E.B.P.; ROSADO, L.D.S.; CORRÊA, R.M. Influência do meio de cultura na germinação de sementes in vitro e taxa de multiplicação de Melissa officinalis L. Revista Ceres, v.55, p.160-167, 2008.
RIVAS, M.A.; FRIERO, I.; ALARCÓN, M.V.; SALGUERO, J. Auxin-cytokinin balance shapes maize root architecture by controlling primary root elongation and lateral root development. Frontiers in Plant Science, v.13, 836592, 2022. https://doi.org/10.3389/fpls.2022.836592
RUAN, J.; YI, P. Exogenous 6-benzylaminopurine inhibits tip growth and cytokinesis via regulating actin dynamics in the moss Physcomitrium patens. Planta, v. 256, n.1, 2022. https://doi.org/10.1007/s00425-022-03914-2
RUTA, C.; LAMBARDI, M.; OZUDOGRU, E. A. Biobanking of vegetable genetic resources by in vitro conservation and cryopreservation. Biodiversity Conservation, v.29, p.3495-3532, 2020. https://doi.org/10.1007/s10531-020-02051-0
SAFITRI, D.C.Y.; HANDINI, E.; APRILIANTI, P.; ISNAINI, Y.; SEMIARTI, E. Improvement of Growth Rate in In Vitro Culture of Paphiopedilum primulinum M. W. Wood & P. Taylor and Paphiopedilum glaucophyllum J. J. Smith using Banana Enrichment Media. Tropical Life Sciences Research, v.35, n.3, p.109-120, 2024. https://doi.org/10.21315/tlsr2024.35.3.5
SASAMORI, M.H.; ENDRES JÚNIOR, D.; DROSTE, A. Optimal conditions for in vitro culture of Cattleya cernua, a small orchid native of Atlantic Forest and Cerrado. Rodriguésia, v.72, e01982019, 2021. https://doi.org/10.1590/2175-7860202172059
SCHAFER, G.; LERNER, B.L. Physical and chemical characteristics and analysis of plant substrate. Ornamental Horticulture, v.28, n.2, p.181- 192, 2022. https://doi.org/10.1590/2447-536X.v28i2.2496
SENDER, J.; RÓŻAŃSKA-BOCZULA, M.; URBAN, D. Active protection of endangered species of peat bog flora (Drosera intermedia, D. anglica) in the Łęczna-Włodawa Lake District. Water, v.14, n.18, p.1- 14, 2022. https://doi.org/10.3390/w14182775
SILVA, M.W.; BARBOSA, L.G.; SILVA, J.E.S.B.; GUIRRA, K.S.; GAMA, D.R.S.; OLIVEIRA, G.M.; DANTAS, B.F. Caracterization of
seed germination of Zephyranthes sylvatica (Mart.) Baker (Amarilidacea). Journal of Seed Science, v.36, n.2, p.178-185, 2014. https://doi.org/10.1590/2317-1545v32n2923
ZAIDI, C.A.; GONZÁLEZ-BENITO, M.E.; PÉREZ-GARCÍA, F. Morphology germination and cryopreservation of seeds of the Mediterranean annual plant Tuberaria macrosepala (Cistaceae). Plant Species Biology, v.25, p.149–157, 2010.
ZARZYCKI, K.; SZELĄG, Z. Red list of the vascular plants in Poland. In: Red list of plants and fungi in Poland. Szafer, W. Kraków: Institute of Botany Polish Academy of Sciences, 2006. p.9-20.
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Copyright (c) 2025 Kamila Antunes Alves, Dayvson Ayala Costa, Miranda Titon, Fernanda Carlota Nery, Paulo Minatell Gonella, Tiago de Oliveira Sousa, Marcela Carlota Nery
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