Karyotype analysis and physical mapping of the 5S and 45S rDNA genes in Hymenocallis howardii (Amaryllidaceae) by FISH
DOI:
https://doi.org/10.1590/2447-536X.v32.e322889Keywords:
fluorescent in situ hybridization, idiogram, ornamental plant bulbous, ribosomal DNAAbstract
Hymenocallis howardii Bauml is a bulbous species of the monocotyledonous Amaryllidaceae family. H. howardii distributes in the Southwest of Mexico. Different chromosome numbers are reported in the genus Hymenocallis, ranging from 24 to 110. Determination of the chromosome number and further cytogenetic analyses are of importance for evolutionary studies and breeding programs. Karyotype analysis and physical mapping of 45S and 5S ribosomal DNA (rDNA) were carried out in meristematic root cells of H. howardii by DAPI staining and fluorescence in situ hybridization (FISH). The 45S rDNA signals were localized in two loci in telomeric position of short arms of chromosomes and six loci for the 5S rDNA signals in telomeric and subtelomeric positions also in short arms of chromosomes. The karyotypic formula is 64m + 30sm + 2st and the karyotype symmetry/asymmetry index is TF % = 40.43, AsK % = 59.56 and Syi % = 67.88 indicating a slightly asymmetric karyotype which showed a gradual decrease in chromosome length from 10.46 µm to 3.36 µm predominating metacentric and submetacentric chromosomes. The localization of only two 45S rDNA signals in the chromosomes of H. howardii suggests that is a paleopolyploid species.
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Copyright (c) 1969 José Manuel Rodríguez Domínguez, Ernesto Tapia Campos, Rodrigo Barba Gonzalez
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