Nanoparticulas de prata eliminam hiperhidricidade em Lavada micropropagada
DOI:
https://doi.org/10.1590/2447-536X.v29i3.2565Palavras-chave:
AgNPs, cultura de tecidos, Lavandula angustifolia Mill., vitrificaçãoResumo
É um grande desafio o cultivo de alfazema fora das condições adequadas. Essa planta, de grande importância econômica, requer condições ótimas para germinar e produzir rendimentos elevados. Sendo o uso da técnica de cultura de tecidos vegetais uma possi- bilidade de melhorar a qualidade da planta. No entanto, um dos principais problemas da micropropagação da lavanda é a ocorrência de hiperhidricidade (HH) devido à presença da citocinina (CK) durante o processo de cultivo in vitro. Dessa forma, objetivou-se realizar um estudo para determinar soluções para a HH em plântulas de lavanda micropropagadas. Diferentes concentrações de na- nopartículas de prata (AgNPs) associados a 1,0 mg de L-1 de 6-benzilaminopurina (BA) foram aplicadas e avaliou-se o desempenho da HH, o crescimento e o desenvolvimento, bem como o teor de fenólicos totais (TPC) e flavonóides totais (TFC). A aplicação de 20 mg L-1 de AgNPs foi considerada a concentração ideal para interromper a ocorrência de HH. Embora a proliferação de brotos tenha sido inferior àquela observada em plantas cultivadas em meio suplementado com BA, a adição dessa concentração de AgNPs melhorou a qualidade dos brotos e das raízes. Acredita-se que o aumento de metabólitos secundários e da atividade antioxidante possa ter contribuído para a resolução da HH.
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