In vitro propagation of alpinia cultivars in different light sources

Abstract

The light source in tissue culture is often one of the punctual factors for the adequacy of plant production. For this, light emitting diodes (LEDs) have been used in order to increase the growth and development of the plants propagated in vitro. The objective was to evaluate the in vitro propagation of cultivars of Alpinia purpurata ‘Red Ginger’ and ‘Pink Ginger’ in two different light sources (fluorescent or LEDs tubes). The experiment was conducted in a completely randomized design, factorial scheme (2x2), totaling four treatments with ten replicates, and the experimental unit composed of one flask containing five explants. After 55 days, white LED and the ‘Red Ginger’ provided the highest number of shoots. For the number of leaves, the cultivar ‘Red Ginger’ and white LED lamps provided superior results. For shoot length (cm), the cultivar ‘Red Ginger’ kept under fluorescent lamps was superior, and ‘Pink Ginger’ presented superiority when cultivated under LED lights. Thus, even with differentiated responses among genotypes, the use of white LED lamps in the in vitro propagation of Alpinia purpurata ‘Red Ginger’ and ‘Pink Ginger’ cultivars is suggested.