Sealing system to improve natural ventilation in culture vessels affects the in vitro development of mother-of-millions (Kalanchoe delagoensis Ecklon and Zeyher)
DOI:
https://doi.org/10.1590/2447-536X.v30.e242734Keywords:
chandelier plant, culture vessel lid, gas-permeable membranes, Kalanchoe, micropropagationAbstract
Sealing systems that provide greater permeability to gasses can favor the development of plants grown in vitro. The objective of this study was to evaluate the effectiveness of a low-cost sealing system that allows different gas exchange [TTCO2] to promote the in vitro growth of mother-of-millions (Kalanchoe delagoensis). Nodal segments of K. delagoensis were disinfected in sodium hypochlorite (2.5%) and inoculated in culture vessels with MS medium, sealed with rigid polypropylene lids with one (1M; TTCO2 21 μL L−1 s −1) or two (2M; TTCO2 25 μL L−1 s −1) gas-permeable membranes. In the control, the culture vessels were sealed with rigid polypropylene lids without membrane (0M; TTCO2 14 μL L−1 s −1). After 45 days of cultivation, growth parameters were measured. Plants kept in culture vessels with 0M and 1M lids had a significant increase in the total length of the plant, main root, aerial part and numbers of nodes when compared to the 2M sealing system. The number of leaves and plantlets along the leaf margin were 35,52%% and 43,69% higher in plants grown in culture vessels with a 1M sealing system. In conclusion, sealing systems that allows gas exchange rates of up to 21 μL L−1 s −1 (0M and 1M) provided the greatest in vitro development of mother-of-millions.
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