In vitro techniques for propagation and breeding of Edible Flowers - recent achievements
DOI:
https://doi.org/10.1590/2447-536X.v31.e312998Keywords:
antioxidants, metabolites, micropropagation, ornamental plants, biotechnology, tissue culture, nutraceutical plantsAbstract
In vitro propagation is widely used in different crops for mass multiplication and the creation of new cultivars. While edible flowers are a part of the ornamental industry, the use of in vitro techniques for these plants has only recently begun. Considering the importance of these species, this review aimed to examine recent advances in the use of in vitro techniques for the propagation and improvement of edible flowers, highlighting their applications, challenges, and recent findings. The number of publications in this field is still limited, with most focusing on shoot proliferation. Callogenesis studies are particularly relevant as they enable the detection, identification, and production of secondary metabolites. Biotechnological approaches, such as genetic transformation and CRISPR, were also identified, though only in a few studies. In conclusion, despite ongoing research, in vitro propagation of edible flowers remains a largely unexplored area, with significant potential as new alternative food and medicinal crop.
Downloads
References
ABOSHAMA, H.M.S. In vitro direct plant regeneration of Calendula officinalis L. Journal of Plant Production, v.30, n.12, p.7955-7966, 2005. https://doi.org/10.21608/jpp.2005.237940
ANDRYS, D.; KULPA, D. In vitro propagation affects the composition of narrow-leaved lavender essential oils. Acta Chromatographica, v.30, n.4, p.225-230, 2017 https://doi.org/10.1556/1326.2017.00317
ANDRYS, D.; KULPA, D.; GRZESZCZUK, M.; BIAŁECKA, B. Influence of jasmonic acid on the growth and antimicrobial and antioxidant activities of Lavandula angustifolia Mill. propagated in vitro. Folia Horticulturae, v.30, n.1, 3-13, 2018. https://doi.org/10.2478/fhort-2018-0001
ASWATHY, J.M.; MURUGAN, K. Micropropagation and genetic fidelity of in vitro grown plantlets of Begonia malabarica Lam. Tropical Life Sciences Research, v.30, n.3, p.37-58, 2019. https://doi.org/10.21315/tlsr2019.30.3.3
BAPAT, V.A.; KAVI KISHOR, P.B.; JALAJA, N.; JAIN, S.M.; PENNA, S. Plant cell cultures: Biofactories for the production of bioactive compounds. Agronomy, v.13, n.858, 2023. https://doi.org/10.3390/agronomy13030858
BELLO, O.A.; ADEDEJI, A.D.; OBEMBE, O.O. Potential utilization of coconut water as a natural substitute to plant growth regulators for in vitro propagation of Hibiscus sabdariffa. International Journal of Agriculture & Biology, v.33, 330215, 2025. https://doi.org/10.17957/IJAB/15.2275
BENYAMMI, R.; KRIMAT, S.; ALILI, M.; BEKHOUCHE, M.; TOUARI, O.; BELALIA, N.; MISSOUM, A.; KHELIFI, L.; MORSLI, A. In vitro propagation of Algerian Lavandula stoechas and assessment of biochemical composition and their antioxidant activity. Bulletin of Pharmaceutical Sciences, v.46, n.2, p.899-911, 2023 https://doi.org/10.21608/bfsa.2023.327719
CATALANO, C.; ABBATE, L.; CARIMI, F.; CARRA, A. GRISTINA, A.S.; PASTA, A.M.S.; GARFI, G. Propagation of Calendula maritima Guss. (Asteraceae) through biotechnological techniques for possible usage in phytotherapy. Agronomy, v.12, 2788, 2022. https://doi.org/10.3390/agronomy12112788
CHEW, L.Y.; TENG, S.K.; NEO, Y.P.; SIM, Y.Y.; CHEW, S.C. The potential of Roselle (Hibiscus sabdariffa) plant in industrial applications: a promising source of functional compounds. Journal of Oleo Science, v.73, p.275-292, 2024. https://doi.org/10.5650/jos.ess23111
CHRYSARGYRIS A, PANAYIOTOU C, TZORTZAKIS N. Nitrogen and phosphorus levels affected plant growth, essential oil composition and antioxidant status of lavender plant (Lavandula angustifolia Mill.). Industrial Crops Production, v.83, p.577-586, 2016. https://doi.org/10.1016/j.indcrop.2015.12.067
CHU, C.C.; WANG, C.C.; SUN, C.S.; HSU, C.; YIN, K.C.; CHU, C.Y.; BI, F.Y. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scientia Sinica, v.18, p.659–668, 1975
COELHO, N.; GONÇALVES, S.; ROMANO, A. Endemic plant species conservation: Biotechnological approaches. Plants, v.9, 345, 2020. https://doi.org/10.3390/plants9030345
DAROUEZ, H.; WERBROUCK, S.P.O. Red and Far-Red Light combined with trans-cinnamic acid enhances in vitro rooting and reduces callus formation in lavender. Horticulturae, v.10, 954, 2024. https://doi.org/10.3390/horticulturae10090954
DAVOUDIPAHNEKOLAYI, M.; DARESTANI, D.N.; MIRSHAHI, H. Multipurpose impacts of silver nitrate on direct organogenesis of Begonia rex cv. DS-EYWA via Transverse Thin Cell Layering (tTCL) technique. Horticulturae, v.10, p.986, 2024. https://doi.org/10.3390/horticulturae10090986
DELTALAB, B.; KAVIANI, B.; KULUS, D.; SAJJADI, S.A. Optimization of shoot multiplication and root induction in Saintpaulia ionantha H. Wendl. using thiamine (vitamin B1) and IBA: a promising approach for economically important African violet propagation. Plant Cell Tissue Organ Culture, v.156, n.74, 2024. https://doi.org/10.1007/s11240-024-02698-5
DOAN, T.T.M.; TRAN, G.H.; NGUYEN, T.K.; LIM, J.H. Antioxidant activity of different cultivars of Chrysanthemum morifolium and quantitative analysis of phenolic compounds by HPLC/UV. Applied Biological Chemistry, v.67, n.17, p.1-13, 2024. https://doi.org/10.1186/s13765-024-00875-w
DOMINGUES, J.; EIRA, A.; RAMALHO, I.; BARROCAS, I.; GONÇALVES, J.C. In vitro propagation and conservation of Lavandula stoechas subsp. Luisieri and Pterospartum tridentatum, two important medicinal and aromatic species from Portugal. Plants, v.13, p.2124, 2024. https://doi.org/10.3390/plants13152124
EMETERE, M.E.; TESTIMONY, G-O. Spectral filtering copper-coated hibiscus-butanol extract for photovoltaic cells. International Journal on Smart Sensing Intelligent Systems, v.13, n.1-6, 2020. https://doi.org/10.21307/ijssis-2020-014
ESCHER, G.B.; BORGES, L.D.C.; SANTOS, J.S.; CRUZ, T.M.; MARQUES, M.B.; CARMO, M.A.V.; AZEVEDO, L.; FURTADO, M.M.; SANT'ANA, A.S., WEN, M.C.; ZHANG, L.; GRANATO, D. From the field to the pot: Phytochemical and functional analyses of Calendula officinalis L. flower for incorporation in an organic yogurt. Antioxidants, v.8, n.11, p.559, 2019 https://doi.org/10.3390/antiox8110559
FATIMA, T.; MUJIB, A.; BANSAL, Y.; DEWIR, Y.H.; MENDLER-DRIENYOVSZKI, N. Indirect organogenesis of Calendula officinalis L. and comparative phytochemical studies of field-grown and in vitro-regenerated tissues. Agronomy, v.14, p.1743, 2024. https://doi.org/10.3390/agronomy14081743
FERNANDES, L.; CASAL, S.; PEREIRA, J.A.; SARAIVA, J.M.A.; RAMALHOSA, E. Edible flowers: A review of the nutritional, antioxidant, antimicrobial properties and effects on human health. Journal of Food Composition and Analysis, v.60, p.38-50, 2017. https://doi.org/10.1016/j.jfca.2017.03.017
FERNANDES, L.; CASAL, S.; PEREIRA, J.A.; SARAIVA, J.M.A.; RAMALHOSA, E. An overview on the market of edible flowers. Food Reviews International, v.36, n.3, p.258-275, 2020. https://doi.org/10.1080/87559129.2019.1639727
GOVINDEN-SOULANGE, J.; BOODIA, N.; DUSSOOA, C.; GUNOWA, R.; DEENSAH, S.; FACKNATH, S.; RAJKOMAR, B. Vegetative propagation and tissue culture regeneration of Hibiscus sabdariffa L. (Roselle). World Journal of Agricultural Sciences, v.5, p.651-661, 2009. http://www.idosi.org/wjas/wjas5(5)/21.pdf
GUBIŠOVÁ, M.; ČIČOVÁ, I. Multiplication of lavender (L. angustifolia) and lavandin (Lavandula x intermedia) in explant culture. Agriculture (Poľnohospodárstvo), v.69, n.1, p.1-12, 2023. https://doi.org/10.2478/agri-2023-0001
HIRUTANI, S., SHIMOMAE, K., YAGUCHI, A. CHIN, D.P.; MII, M.; IGAWA, T. Efficient plant regeneration and Agrobacterium-mediated transformation of Begonia semperflorens-cultorum. Plant Cell Tissue Organ Culture, v.142, p.435-440, 2020. https://doi.org/10.1007/s11240-020-01858-7
JIMÉNEZ-MARIÑA LIUDMILA. Propagation methodology in vitro of Dahlia sp. Avances, v.22, n.3, p.406-422, 2020. Available at: https://www.redalyc.org/articulo.oa?id=637869117007
JURAEV, Z., KORIYEV, M. R., SHOKIROVA, G. In vitro micropropagation for standardizing medicinal plants: a systems-based case study of Hibiscus sabdariffa and Glycyrrhiza glabra. In Vitro Cellular & Developmental Biology – Plant, v.61, n.4, p.552–563, 2025 https://doi.org/10.1007/s11627-025-10557-x
KASSA, B.A.; MEKBIB, F.; ASSEFA, K. Effects of plant hormones and genotypes on anther culture response of safflower (Carthamus tinctorius L.). Scientific African, v. 26, e02367, 2024. https://doi.org/10.1016/j.sciaf.2024.e02367
KIYMAZ, G.; ACEMI, A. In vitro propagation of ornamental snapdragon (Antirrhinum majus L.) revisited: an analysis on the effects of plant growth regulators. Commagene Journal of Biology, v.8, n.1, p.37-45, 2024. https://doi.org/10.31594/commagene.1450579
KOEFENDER, J.; MANFIO, C.E.; CAMERA, J.N.; SCHOFFEL, A.; GOLLE, D.P. Micropropagation of lavender: a protocol for production of plantlets. Horticultura Brasileira, v.39, p.404-410, 2021. http://dx.doi.org/10.1590/s0102-0536-20210409
KUMAR, S.S.; MANOJ, P.; GIRIDHAR, P. Micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (Hibiscus sabdariffa L.). In Vitro Cellular & Developmental Biology-Plant, v.52, p.427–436, 2016. https://doi.org/10.1007/s11627-016-9785-2
KUMAR, K.R.; SINGH, K.P.; BHATIA, R.; RAJU, D.V.S.; PANWAR, S. Optimising protocol for successful development of haploids in marigold (Tagetes spp.) through in vitro androgenesis. Plant Cell Tissue and Organ Culture, v.138, n.2-3, p.11-28, 2019. https://doi.org/10.1007/s11240-019-01598-3
KUMAR, S.; UTTAM, A.; SHARMA, S.; KUMAR, V. Edible vegetable flowers: Next generation sustainable super foods, therapeutic role, processing and improvement approaches. International Journal of Gastronomy and Food Science, v.39, 101116, 2025. https://doi.org/10.1016/j.ijgfs.2025.101116
KWON, J.-H.; OH, H-J.; LEE, D.-S.; IN, S-J.; SEO, K.-H.; JUNG, J.-W.; CHA, B.-J.; LEE, D.Y.; BAEK, N.-I. Pharmacological activity and quantitative analysis of flavonoids isolated from the flowers of Begonia semperflorens Link et Otto. Applied Biological Chemistry, v. 62, n.1, p 11, 2019. https://doi.org/10.1186/s13765-019-0416-6
LARA, C.F.; PAIVA, P.D.O.; SOUZA, R.R.; CUNHA NETO, A.R.; REIS, M.V.; GUILHERME, L.R.G. Selenium biofortification of Tropaeolum majus L. Bragantia, v.84, 2025. https://doi.org/10.1590/1678-4499.20240256
LI, G.; MICHAELIS, D.F.; HUANG, J.; SEREK, M.; GEHL, C. New insights into the genetic manipulation of the R2R3-MYB and CHI gene families on anthocyanin pigmentation in Petunia hybrida. Plant Physiology and Biochemistry, v.203, 108000, 2023. https://doi.org/10.1016/j.plaphy.2023.108000
LIM, T.K. Edible medicinal and non-medicinal plants, Vol 7: Flowers. Springer:Dordrecht, 2014. 1102p. https://doi.org/10.1007/978-94-007-7395-0
MACHADO, M.P.; CIOTTA, M.N.; DESCHAMPS, C.; ZANETTE, F.; CÔCCO, L.C.; BIASI, L.A. Propagação in vitro e caracterização química do óleo essencial de Lavandula angustifolia cultivada no Sul do Brasil. Ciência Rural, v.43, p.283-289, 2013. https://doi.org/10.1590/S0103-84782013000200015
MEHBUB, H., AKTER, A., AKTER, M.A., MANDAL, M.S.H., HOQUE, M.A., TULEJA, M., MEHRAJ, H. Tissue culture in ornamentals: cultivation factors, propagation techniques, and its application. Plants, v.11, n.23, 3208, 2022. https://doi.org/10.3390/plants11233208
MURASHIGE, T., SKOOG, F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, v.15, n.3, p.473-497, 1962. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
MOURA, R.C.; SANTOS, J.P.; ASSIS, R.M.A.; ROCHA, J.P.M.; LEITE, J.J.F; PEREIRA, F.D.; BERTOLUCCI, S.K.V.; PINTO, J.E.B.P. Application of selenite and selenate sources in the micropropagation of Digitalis mariana Boiss. ssp. Heywoodii. Research, Society and Development, v.12, n.1, p.e17112139703, 2023. https://doi.org/10.33448/rsd-v12i1.39703
NABIEVA, A.Y.; FERSHALOVA, T.D. A novel approach for begonias micropropagation by inflorescence explants. Ornamental Horticulture, v.29, n.4, p.462-470, 2023. https://doi.org/10.1590/2447-536X.v29i4.2595
NAKANO, M., HOSHINO, Y., MII, M. Somatic hybridization in Dianthus species. In: NAGATA, T., BAJAJ, Y.P.S. (eds) Somatic hybridization in crop improvement II. Biotechnology in Agriculture and Forestry, vol 49. Berlin, Heidelberg: Springer, 2001. https://doi.org/10.1007/978-3-642-56758-2_19
NARAYANAN, Z.; GLICK, B.R. Biotechnologically engineered plants. Biology, v.12, p.601, 2023. https://doi.org/10.3390/biology12040601
NISHIHARA, M.; HIRABUCHI, A.; TESHIMA, T.; UESUGI, S.; TAKAHASHI, H. Flower color modification in Torenia fournieri by genetic engineering of betacyanin pigments. BMC Plant Biology, v.24, 614, 2024. https://doi.org/10.1186/s12870-024-05284-1
OMOLE, U.; ORANUSI, S. Wine production from Hibiscus sabdariffa Calyxes using probiotics starter cultures. IOP Conference Series: Earth and Environmental Science, n.331:012066, 2019. https://doi.org/10.1088/1755-1315/331/1/012066
OMOLE, U.; ORANUSI, S.; AHUEKWE, E.F.; KADE, A. The physicochemical and microbiological characteristics of fermented Hibiscus sabdariffa Calyxes using probiotics starter cultures. Tropical Journal of Natural Product Research, v.6, n.4, p.580-586, 2022.
OZDEN-TOKATLI, Y.; DE CARLO, A.; GUMUSEL, F.; PIGNATELLI, S; LAMBARDI, M. Development of encapsulation techniques for the production and conservation of synthetic seeds in ornamental plants. Propagation Ornamental Plants, v.8, n.1, p.17-22, 2008.
OZUDOGRU, E.A.; PREVIATI, A.; LAMBARDI, M. In vitro conservation and cryopreservation of ornamental plants. Methods Molecular Biology, v.589, p.303-324, 2010. https://doi.org/10.1007/978-1-60327-114-1_28
PAIVA, P.D.O. Climate change as a challenge for Floriculture. Ornamental Horticulture, v.29, n.1, p.6, 2023. https://doi.org/10.1590/2447-536X.v.29i1.2590
PAIVA, P.D.O.; CASTRO, A.C.R.; VAN DER GEEST, A.P.S.L.; VIEIRA, G.F.R.; HUMMEL, M.; LOPES, H.S.; COSTA, J.L.S. Diagnosis of the production chain of flowers, turfgrass, and ornamental plants: proposal for an innovation agenda. Ornamental Horticulture, v.30, e242792, 2024. https://doi.org/10.1590/2447-536X.v30.e242792
ROUT, G.R., MOHAPATRA, A., JAIN, S.M. Tissue culture of ornamental pot plant: A critical review on present scenario and future prospects. Biotechnology Advances, v.24, n.6, p.531-560, 2006. https://doi.org/10.1016/j.biotechadv.2006.05.001
SANTOS, I.C.; REIS, S.N. Edible flowers: Traditional and current use. Ornamental Horticulture, v.27, n.4, p.438-445, 2021. https://doi.org/10.1590/2447-536X.v27i4.2392
SARODE, D.K.; PAGARIYA, M.C.; JADHAV, P.R.; PATIL, S.A.; DEVARUMATH, R.M.; SHINGOTE, P.R.; PRASAD, K.V.; JAIN, S.M.; PENNA, S.; KAWAR, P.G. Edible flowers: biotechnological interventions for improving bioactives of food and health significance. Journal of Food Composition and Analysis, v.134, 106506. 2024. https://doi.org/10.1016/j.jfca.2024.106506
SCARIOT, V.; FERRANTE, A.; ROMANO, D. Edible flowers: Understanding the effect of genotype, preharvest, and postharvest on quality, safety, and consumption. Frontiers in Plant Science, v.13, p.1025196, 2022. https://doi.org/10.3389/fpls.2022.1025196
SCHWINN, K.E.; BOASE, M.R.; BRADLEY, J.M.; LEWIS, D.H.; DEROLES, S.C.; MARTIN, C.R.; DAVIES, K.M. MYB and bHLH transcription factor transgenes increase anthocyanin pigmentation in petunia and lisianthus plants, and the petunia phenotypes are strongly enhanced under field conditions. Frontiers in Plant Science, v.5, p.111596, 2014. https://doi.org/10.3389/fpls.2014.00603
SOOD, Y.; LAL, M.; KALIA, A.; VERMA, S. Edible flowers: super foods with potential health benefits. International Journal of Plant & Soil Science, v.36, n.3, p.213-221, 2024. https://doi.org/10.9734/ijpss/2024/v36i34417
SUPRASANNA, P.; JAIN, S.M. Biotechnology and induced mutations in ornamental plant improvement. Acta Horticulturae, n.1334, 2022. https://doi.org/10.17660/ActaHortic.2022.1334.1
VARGAS, D.A.; VARGAS, N.; OSORIO-DOBLADO, A.M.; RUANO-ORTIZ, J.A.; MEDEIROS, F.G.M.; HOSKIN, R.T.; MONCADA, M. Valorization of hibiscus flower (Hibiscus sabdariffa L.) anthocyanins to produce sustainable spray-dried ingredients. Sustainability, v.16, n.13, p.5523. https://doi.org/10.3390/su16135523
VICTORIO, C.P.; LAGE, C.L.; SATO, A. Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis. Revista Ciência Agronômica, v.43, n.3, 539-545. https://doi.org/10.1590/S1806-66902012000300017
VRANCHEVA, R.; IVANOV, I.; ANEVA, I.; STOYANOVA, M.; PAVLOV, A. Food additives and bioactive substances from in vitro systems of edible plants from the Balkan peninsula. Engineering in Life Sciences, v.18, n.11, p.799-806, 2018. https://doi.org/10.1002/elsc.201800063
WANG, M.R.; CUI, Z.H.; LI, J.W.; HAO, X-Y.; ZHAO, L.; WANG, Q-C. In vitro thermotherapy-based methods for plant virus eradication. Plant Methods, v.14, n.87, 2018. https://doi.org/10.1186/s13007-018-0355-y
YAHYA, M.A.; TUNALI, F.; KILLI, D.; SÖKMEN, A. Phenolic profile and volatiles of in vitro propagated Lavandula angustifolia Mill. seedlings. Python - International Journal of Experimental Botany, v.93, n.3, p.427-444, 2024. https://doi.org/10.32604/phyton.2024.046271
ZHANG, C.; FENG, M.; CHITRAKAR, B.; YANG, F.; WEI, B.; WANG, B.; ZHOU, C.; MA, H.; GAO, X.; XU, B. In Vitro inhibitory mechanism of polyphenol extracts from multi-frequency power ultrasound-pretreated rose flower against α-Glucosidase. Foods, v.13, p.3421, 2024. https://doi.org/10.3390/foods13213421
ZHANG, Q.; CHENG, Z.; FAN, Y. ZHANG, D.; WANG, M.; ZHANG, J.; SOMMANO, S.; WU, X.; LONG, C. Ethnobotanical study on edible flowers in Xishuangbanna, China. Journal of Ethnobiology and Ethnomedicine, v.19, n.1, p.43, 2023. https://doi.org/10.1186/s13002-023-00608-1
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Patrícia Duarte de Oliveira Paiva, Anderson Condé da Silva, Renato Paiva, Margherita Irene Beruto

This work is licensed under a Creative Commons Attribution 4.0 International License.





