Análise morfológica e da diversidade genética baseada em SRAP de acessos de Zinnia elegans Jacq.
DOI:
https://doi.org/10.1590/2447-536X.v31.e312949Palavras-chave:
marcador molecular, planta ornamental, filogenia, variabilidade, acessoResumo
Zinnia elegans Jacq. é uma planta ornamental comumente cultivada na Indonésia. A espécie possui vários formatos e cores de flores e é fácil de ser cultivada em quaisquer condições ambientais. Este estudo teve como objetivo determinar a diversidade genética de 18 acessos de Z. elegans com base em marcadores morfológicos e moleculares SRAP. A pesquisa foi organizada em um delineamento inteiramente casualizado (CRD) com 18 acessos como tratamentos e três repetições dentro de cada tratamento. A análise SRAP foi realizada usando cinco combinações de primers de C1 (Me6-Em6), C2 (Me8-Em4), C3 (Me8-Em7), C4 (Me9-Em4) e C5 (Me10-Em4). Enquanto isso, o descritor Z. elegans da UPOV foi usado na análise morfológica para características quantitativas e qualitativas. O resultado mostrou um dendrograma com dois ramos principais do grupo A e B para características morfológicas e marcadores SRAP com um coeficiente de similaridade de 0,18-0,78 e 0,46-0,80, respectivamente. Os marcadores SRAP correlacionaram-se significativamente com seis características morfológicas, como ápice do florete radial (RFA), comprimento do florete radial (RFL), comprimento da folha (LL), largura da folha (LW), comprimento do caule (SL) e largura do florete radial (RFW). Cinco características morfológicas que apresentaram alto VGC e forte correlação com SRAP foram priorizadas para seleção. Cinco primers de combinação SRAP podem ser usados como marcadores moleculares para o melhoramento de zínias.
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ABD EL-FATAH, B.; NAFEA, D. Genetic relationships and diversity among pea (Pisum sativum L.) genotypes assessed using agro- morphological and molecular markers. Journal of Agricultural Chemistry and Biotechnology, v.11, n.12, p.353-363, 2020. https://doi.org/10.21608/jacb.2020.137305
ABDOLINASAB, M.; RAHIMI, M. Association analysis of traits in watermelon genotypes using molecular markers. Iranian Journal of Science and Technology, Transaction A: Science, v.44, p.361-369, 2020. https://doi.org/10.1007/s40995-020-00837-z
AMIRYOUSEFI, A.; HYVÖNEN, J.; POCZAI, P. iMEC: Online marker efficiency calculator. Applications in Plant Sciences, v.6, n.6, p.1-4, 2018. https://doi.org/10.1002/aps3.1159
ANNEPU, S.K.; SHARMA, H.D.; BARH, A.; DOGRA, R.; SHARMA, V.; THAKUR, S.; VERMA, V.; SHARMA, K. Performance prediction of F1 crosses in eggplant (Solanum melongena L.) based on morphological and molecular divergence. Genetika, v.55, n.1, p.45-60, 2023. https://doi.org/10.2298/GENSR2301045A
BIDYANANDA, N.; JAMIR, I.; NOWAKOWSKA, K.; VARTE, V.; VENDRAME, W.A.; DEVI, R.S.; NONGDAM, P. Plant genetic diversity studies: insights from DNA marker analyses. International Journal of Plant Biology, v.15, p.607-640, 2024. https://doi.org/10.3390/ijpb15030046
CHESNOKOV, Y.V.; ARTEMYEVA, A.M. Evaluation of the measure of polymorphism information of genetic diversity. Agricultural Biology, v.50, n.5, p.571-578, 2015. https://doi.org/10.15389/agrobiology.2015.5.571eng
CORNEA-CIPCIGAN, M.; PAMFIL, D.; SISEA, C.R.; MARGAOAN, R. Characterization of Cyclamen genotypes using morphological descriptors and DNA molecular markers in a multivariate analysis. Frontiers in Plant Science, v.14, p.1-15, 2023. https://doi.org/10.3389/fpls.2023.1100099
DARKWA, K.; AGRE, P.; OLASANMI, B.; ISEKI, K.; MATSUMOTO, R.; POWELL, A.; BAUCHET, G.; DE KOEYER, D.; MURANAKA, S.; ADEBOLA, P.; ASIEDU, R.; TERAUCHI, R.; ASFAW, A. Comparative assessment of genetic diversity matrices and clustering methods in white Guinea yam (Dioscorea rotundata) based on morphological and molecular markers. Scientific Reports, v.10, p.1-14, 2020. https://doi.org/10.1038/s41598-020-69925-9
DESHMUKH, S.N.; BASU, M.S.; REDDY, P.S. Genetic variability, character association and path coefficient analysis of quantitative traits in Virginia bunch varieties of groundnut. Indian Journal of Agricultural Science, v.56, p.515-518, 1986.
DOYLE, J.J.; DOYLE, J.L. Isolation of Plant DNA from fresh tissue. Focus, v.12, n.1, p.13–15, 1990.
FU, X.P.; NING, G.G.; GAO, L.P.; BAO, M.Z. Genetic diversity of Dianthus accessions as assessed using two molecular marker systems (SRAPs and ISSRs) and morphological traits. Scientia Horticulturae, v.117, n.3, p.263-270, 2008. https://doi.org/10.1016/j.scienta.2008.04.001
GOLKAR, P.; NOURBAKHSH, V. Analysis of genetic diversity and population structure in Nigella sativa L. using agronomic traits and molecular markers (SRAP and SCoT). Industrial Crops and Products, v.130, p.170-178, 2019. https://doi.org/10.1016/j.indcrop.2018.12.074
HARRIS, A.M.; DEGIORGIO, M. An unbiased estimator of gene diversity with improved variance for samples containing related and inbred individuals of any ploidy. G3: Genes, Genomes, Genetics, v.7, p.671-691, 2017. https://doi.org/10.1534/g3.116.037168
HASSANI, S.M.R.; TALEBI, R.; POURDAD, S.S.; NAJI, A.M.; FAYAZ, F. Morphological description, genetic diversity and population structure of safflower (Carthamus tinctorius L.) mini core collection using SRAP and SSR markers. Biotechnology and Biotechnological Equipment, v.34, n.1, p.1043-1055, 2020. https://doi.org/10.1080/13102818.2020.1818620
HODAEI, M.; RAHIMMALEK, M.; ARZANI, A. Genetic diversity of Iranian Chrysanthemum morifolium cultivars using morphological traits and sequence-related amplified polymorphism (SRAP) markers. Horticulture Environment and Biotechnology, v.60, p.753-765, 2019. https://doi.org/10.1007/s13580-019-00137-5
JADHAV, P.R.; JAGTAP, A.Y.; GADGE, A.D.; SOLANKE, A.U.; PAGARIYA, M.C.; KADAM, G.B.; PRASAD, K.V.; KAWAR, P.G. Agromorphological characterization and SRAP-based genetic diversity analysis of Gladiolus hybridus L. cultivars. Genetic Resources Crop Evolution, p.1-23, 2025. https://doi.org/10.1007/s10722-024-02309-4
KHALED, A.G.A.; ELAMEEN, T.M.; AHMED, A.Y.M.; MOHIY, M.; ELSHAZLY, I.F.O. SRAP molecular markers linked to three morphological traits in Egyptian Bread Wheat (Triticum aestivum L.). SVU-International Journal of Agricultural Sciences, v.3, n.3, p.145-158, 2021. https://doi.org/10.21608/svuijas.2021.78975.1113
LI, G.; QUIROS, C.F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: Its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics, v.103, n.2, p.455-461, 2001. https://doi.org/10.1007/s001220100570
LIU, C.; HE, Y.; GOU, T.; LI, X.; NING, G.; BAO, M. Identification of molecular markers associated with the double flower trait in Petunia hybrida. Scientia Horticulturae, v.206, p.43-50, 2016. https://doi.org/10.1016/j.scienta.2016.04.032
MARETTA, D.; SOBIR; HELIANTI, I.; PURWONO; SANTOSA, E. Genetic diversity in eddoe taro (Colocasia esculenta var. antiquorum) from Indonesia based on morphological and nutritional characteristics. Biodiversitas, v.21, n.8, p.3525-3533, 2020. https://doi.org/10.13057/biodiv/d210814
MARTINS, R.D.C.F.; PÊGO, R.G.; DA CRUZ, E.S.; ABREU, J.F.G.; DE CARVALHO, D.F. Production and quality of zinnia under different growing seasons and irrigation levels. Ciencia e Agrotecnologia, v.45, p.1-13, 2021. https://doi.org/10.1590/1413-7054202145033720
MAULANI, R.; MURTI, R.H.; PURWANTORO, A. Molecular diversity in populations of Chili (Capsicum annuum L.). Sabrao Journal of Breeding and Genetics, v.55, n.1, p.15-24, 2023. https://doi.org/10.54910/sabrao2023.55.1.2
NAKKUNTOD, M.; LUANGLUE, S. Genetic diversity of Nelumbo nucifera Gaertn. cultivars using DNA markers and morphological traits. Chiang Mai Journal of Science, v.51, n.6, p.1-16, 2024. https://doi.org/10.12982/CMJS.2024.087
PONGOH, J.; PAAT, F.J.; SOPUTAN, R. The diversity of several flower color types of the zinnia plant (Zinnia elegans Jacq.). Jurnal Agroekoteknologi Terapan (Applied Agroecotechnology Journal), v.3, n.1, p.108-115, 2022. https://doi.org/10.35791/jat.v3i1.41110
SHARAF-ELDIEN, M.; EL-BABLY, S.; MAGOUZ, M. Effect of pinching and spraying of paclobutrazol on vegetative growth, flowering and chemical composition of Zinnia elegans Jacq. Journal of Plant Production, v.8, n.5, p.587-592, 2017. https://doi.org/10.21608/jpp.2017.40474
SINGH, A.; DIKSHIT, H.K.; JAIN, N.; SINGH, D.; YADAV, R.N. Efficiency of SSR, ISSR and RAPD markers in molecular characterization of mungbean and other vigna species. Indian Journal of Biotechnology, v.13, n.1, p.81-88, 2014.
SLEPER, D.A.; POEHLMAN, J.M. Breeding Field Crop (5th ed.). Oxford: Blackwell Publishing, 2006. 432p.
SONG, C.; LIU, X.; XU, M.; YING, M.; FU, J.; ZHANG, C. Germplasm resource and genetic breeding of Zinnia: a review. Ornamental Plant Research, v.5, p.1-10, 2025. https://doi.org/10.48130/opr-0025-0014
SYUKUR, M.; SUJIPRIHATI, S.; YUNIANTI, R. Teknik Pemuliaan Tanaman (2nd ed.). Indonesia: Penebar Swadaya, 2015. 354p.
TOSCANO, S.; ROMANO, D. Morphological, physiological, and biochemical responses of zinnia to drought stress. Horticulturae, v.7, v.362, p.1-17, 2021. https://doi.org/10.3390/horticulturae7100362
UPOV. Zinnia: Guidelines for the conduct of tests for distinctness, uniformity and stability, 2022. Available at: http://www.Upov.Int/Edocs/Upov Accessed on: August 18th 2024.
WU, F.; CHEN, J.; WANG, J.; WANG, X.; LU, Y.; NING, Y.; LI, Y. Intra-population genetic diversity of Buchloe dactyloides (Nutt.) Engelm (buffalograss) determined using morphological traits and sequence-related amplified polymorphism markers. 3 Biotech, v.9, n.97, p.1-8, 2019. https://doi.org/10.1007/s13205-019-1632-9
ZHANG, Z.; YANG, Q.; NIU, Y.; ZHANG, Y.; DONG, S.; ZHANG, W.; WANG, Z. Diversity analysis and establishment of core collection among Akebia trifoliata (Thunb.) Koidz. in Qinba mountain area of China using ISSR and SRAP markers. Genetic Resources and Crop Evolution, v.68, p.1085-1102, 2021. https://doi.org/10.1007/s10722-020-01051-x
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Copyright (c) 2025 Miranda Ferwita Sari, Aziz Purwantoro, Yekti Asih Purwestri, Endang Sulistyaningsih
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
