O 24-epibrassinolídeo melhora a qualidade e a defesa antioxidante em flores cortadas de Dendrobium ‘Khao Sanan’
DOI:
https://doi.org/10.1590/2447-536X.v32.e322970Palavras-chave:
Antioxidantes, brassinosteroides, flor de corte, peroxidação lipídica, senescência floralResumo
A longevidade de flores de corte é influenciada pela regulação hormonal, entre os quais os brassinosteroides (BRs) desempenham um papel fundamental na melhoria da qualidade pós-colheita de culturas florícolas. No entanto, o impacto dos BRs em orquídeas Dendrobium de corte permanece inexplorado. Este estudo teve como objetivo investigar os efeitos do 24-epibrassinolídeo (EBL) sobre as respostas fisiológicas e a senescência de flores de corte de Dendrobium ‘Khao Sanan’. Os botões florais foram imersos em soluções de EBL nas concentrações de 2,5, 5, 10 e 20 µM durante 1 h, enquanto água destilada foi utilizada como tratamento controle (0 µM). Subsequentemente, todas as flores foram colocadas em água destilada e mantidas a 25 °C. A qualidade floral e os índices de abertura foram determinados por observações morfológicas. Além disso, alterações fisiológicas foram avaliadas pela mensuração da produção de etileno, taxa respiratória, conteúdo de peróxido de hidrogênio (H2O2), peroxidação lipídica e atividades e capacidade de enzimas antioxidantes durante e ao final do armazenamento. Os resultados mostraram que flores tratadas com 2,5 e 5 µM de EBL apresentaram produção de etileno, taxa respiratória, conteúdo de H2O2 e atividade da lipoxigenase (LOX) substancialmente menores, juntamente com redução do conteúdo de malondialdeído (MDA). Além disso, flores tratadas com 2,5 e 5 µM de EBL apresentaram atividades significativamente maiores de catalase (CAT) e ascorbato peroxidase (APX), bem como maior capacidade antioxidante do que os demais tratamentos, enquanto flores tratadas com 20 µM de EBL exibiram respostas adversas, incluindo aceleração da senescência, aumento dos danos oxidativos e redução da qualidade floral. Este estudo indica que baixas concentrações de EBL retardaram a senescência floral por meio do aumento da capacidade antioxidante, enquanto altas concentrações aceleraram a senescência. Portanto, orquídeas de corte Dendrobium ‘Khao Sanan’ responderam diferencialmente ao EBL de maneira dependente da concentração.
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Copyright (c) 2026 Chawisa Sukpitak, Kanogwan Seraypheap
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