Dendrobium nobile in vitro flowering induction
DOI:
https://doi.org/10.1590/2447-536X.v29i2.2612Keywords:
flowering, temperature, thidiazuron, OrchidaceaeAbstract
In vitro flowering is a technique used in genetic improvement that accelerates generations and favors the faster fixation of new traits of agronomic and market interest. The study aimed to establish a protocol for the in vitro flowering of Dendrobium nobile, through the combined temperature and of the growth regulator thidiazuron (TDZ) effects. Experiments was performed in a flask that was kept in a BOD incubator at 18, 21, or 24 °C or in a growth room at 26 °C. The TDZ concentrations were 0.0, 0.5, 1.0, and 2.0 mg L-1. The highest percentage of flowering shoots of 13.7% occurred at a concentration of 2 mg L-1 of TDZ grown at a temperature of 18 °C. Dendrobium nobile in vitro flowering was promoted in plants cultivated in ½ MS supplemented with 30 g L-1 sucrose, 5.5 g L-1 agar, 100 mL L-1 coconut water, pH 6.0, and 2.0 mg L-1 TDZ at a controlled temperature of 18 °C and a photoperiod of 16 h. The in vitro flowering induction protocol of the D. nobile species could be used or improved for future studies.
Downloads
References
BOTELHO, F.B.S.; RODRIGUES, C.S.; BRUZI, A.T. Ornamental plant breeding. Ornamental Horticulture, v.21, n.1, p.9-16, 2015. https://doi.org/10.14295/rbho.v21i1.770
BRIDGEN, M.P.; VAN HOUTVEN, W.; EECKHAUT, T. Plant tissue culture techniques for breeding. In: VAN HUYLENBROECK, J. (eds). Ornamental Crops. Handbook of Plant Breeding, Cham: Springer, 2018. p.127-144.
CAMPOS, K.O.; KERBAUY, G.B. Thermoperiodic effect on flowering and endogenous hormonal status in Dendrobium (Orchidaceae). Journal of Plant Physiology, v.161, n.12, p.1385-1387, 2004. https://doi.org/10.1016/j.jplph.2004.07.008
CHANDLER, J. W. The Hormonal regulation of flower development. Journal of Plant Growth Regulation, v. 30, n. 2, p. 242–254, 2011.
DINANI, E.T.; SHUKLA, M.R.; TURI, C.E.; SULLIVAN, J.A.; SAXENA, P.K. Thidiazuron: modulator of morphogenesis in vitro. In: AHMAD, N.; FAISAL, M. (eds). Thidiazuron: From Urea Derivative to Plant Growth Regulator. Singapore: Springer, 2018. p.1-36.
ENG, W.-H.; HO, W.-S. Polyploidization using colchicine in horticultural plants: A review. Scientia Horticulturae, v.246, p.604–617, 2019. https://doi.org/10.1016/j.scienta.2018.11.010
HUYLENBROECK, J. VAN; BHATTARAI, K. Ornamental plant breeding: entering a new era? Ornamental Horticulture, v.28, n.3, p.297-305, 2022. https://doi.org/10.1590/2447-536x.v28i3.2516
KAUR, S. In vitro florigenesis with special reference to Orchids - A review. Recent Patents on Biotechnology, v.16, n.4, p.311-318, 2022. https://doi.org/10.2174/1872208316666220415124439
KNUDSON, L. A new nutrient solution for the germination of orchid seed. American Orquid Society Bulletim, v.14, p.214-217, 1946.
LI, S.; ZHOU, J.; XU, S.; LI, J.; LIU, J.; LU, Y.; SHI, J.; ZHOU, S.; WU, Q. Induction of Nrf2 pathway by Dendrobium nobile Lindl. alkaloids protect against carbon tetrachloride induced acute liver injury. Biomedicine and Pharmacotherapy, v.117, p.109073, 2019. https://doi.org/10.1016/j.biopha.2019.109073
MOHAMMADI, M.; KAVIANI, B.; SEDAGHATHOOR, S. In vivo polyploidy induction of Phalaenopsis amabilis in a bubble bioreactor system using colchicine. Ornamental Horticulture, v.27, n.2, p.204-212, 2021. https://doi.org/10.1590/2447-536x.v27i2.2275
MURASHIGE, T., SKOOG, F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, v.15, p.473-497, 1962. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
NOHALES, M.A.; KAY, S.A. Gigantea gates gibberellin signaling through stabilization of the DELLA proteins in Arabidopsis. Proceedings of the National Academy of Sciences, v.116, n.43, p.21893-21899, 2019. https://doi.org/10.1073/pnas.1913532116
PUJARI, I.; SANKAR BABU, V. Precocious in vitro flowering in threatened ornamental orchid, Dendrobium ovatum – Decoding the causal factors. Current Plant Biology, v.31, p.100257, 2022. https://doi.org/10.1016/j.cpb.2022.100257
REFLORA. (2020). Herbário Virtual. Available at: <https://reflora.jbrj.gov.br/reflora/herbarioVirtual/> Accessed on: January 30th 2023.
SARSAIYA, S.; JAIN, A.; JIA, Q.; FAN, X.; SHU, F.; CHEN, Z.; ZHOU, Q.; SHI, J.; CHEN, J. Molecular identification of endophytic fungi and their pathogenicity evaluation against Dendrobium nobile and Dendrobium officinale. International Journal of Molecular Sciences, v.21, n.1, p.1-16, 2020. https://doi.org/10.3390/ijms21010316
SILVA, J.A.T.; KERBAUY, G.B.; ZENG, S.; CHEN, Z.; DUAN, J. In vitro flowering of orchids. Critical Reviews in Biotechnology, v.34, n.1, p.56-76, 2014. https://doi.org/10.3109/07388551.2013.807219
SILVA, J.A.T.D.A; NG, T.B. The medicinal and pharmaceutical importance of Dendrobium species. Applied Microbiology and Biotechnology, v.101, n.6, p.2227-2239, 2017. https://doi.org/10.1007/s00253-017-8169-9
TAIZ, L.; ZEIGER, E.; MOLLER, I. M.; MURPHY, A. Plant Physiology and Development. 6ed. Sunderland: Sinauer Associates, Inc., Publishers. 2017. 888p.
WANG, S.-L.; AN, H. R.; TONG, C.-G.; JANG, S. Flowering and flowering genes: from model plants to orchids. Horticulture, Environment, and Biotechnology, v.62, n.2, p.135-148, 2021. https://doi.org/10.1007/s13580-020-00309-8
WANG, S.-L.; VISWANATH, K.K.; TONG, C.-G.; AN, H.R.; JANG, S.; CHEN, F.-C. Floral induction and flower development of orchids. Frontiers in Plant Science, v.10, p. 1-15, 2019. https://doi.org/10.3389/fpls.2019.01258
WANG, Z.H.; WANG, L.; YE, Q.S. High frequency early flowering from in vitro seedlings of Dendrobium nobile. Scientia Horticulturae, v.122, n.2, p.328-331, 2009. https://doi.org/10.1016/j.scienta.2009.05.018
WEN, Z.; GUO, W.; LI, J.; LIN, H.; HE, C.; LIU, Y.; ZHANG, Q.; LIU, W. Comparative transcriptomic analysis of vernalization- and cytokinin-induced floral transition in Dendrobium nobile. Scientific Reports, v.7, p.45748, 2017. https://doi.org/10.1038/srep45748
ZHANG, D.; LIAO, Y.; LU, S.; LI, C.; SHEN, Z.; YANG, G.; YIN, J. Effect of thidiazuron on morphological and flowering characteristics of Dendrobium ‘Sunya Sunshine’ potted plants. New Zealand Journal of Crop and Horticultural Science, v.47, n.3, p.170-181, 2019. https://doi.org/10.1080/01140671.2019.1576744
ZHAO, D.; HU, G.; CHEN, Z.; SHI, Y.; ZHENG, L.; TANG, A.; LONG, C. Micropropagation and in vitro flowering of Dendrobium wangliangii: A critically endangered medicinal orchid. Journal of Medicinal Plants Research, v.7, n.28, p.2098-2110, 2013. https://doi.org/10.5897/JMPR11.1777
ZHENG, J.; MA, Y.; ZHANG, M.; LYU, M.; YUAN, Y.; WU, B. Expression pattern of FT/TFL1 and miR156- Targeted SPL genes associated with developmental stages in Dendrobium catenatum. International Journal of Molecular Sciences, v.20, n.11, p.2725, 2019. https://doi.org/10.3390/ijms20112725
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Michele Carla Nadal, Gracielle Vidal Silva Andrade, José Henrique Nunes Flores, Michele Valquíria dos Reis, Vantuil Antonio Rodrigues, Moacir Pasqual
This work is licensed under a Creative Commons Attribution 4.0 International License.
