Dendrobium nobile in vitro flowering induction

Authors

DOI:

https://doi.org/10.1590/2447-536X.v29i2.2612

Keywords:

flowering, temperature, thidiazuron, Orchidaceae

Abstract

In vitro flowering is a technique used in genetic improvement that accelerates generations and favors the faster fixation of new traits of agronomic and market interest. The study aimed to establish a protocol for the in vitro flowering of Dendrobium nobile, through the combined temperature and of the growth regulator thidiazuron (TDZ) effects. Experiments was performed in a flask that was kept in a BOD incubator at 18, 21, or 24 °C or in a growth room at 26 °C. The TDZ concentrations were 0.0, 0.5, 1.0, and 2.0 mg L-1. The highest percentage of flowering shoots of 13.7% occurred at a concentration of 2 mg L-1 of TDZ grown at a temperature of 18 °C. Dendrobium nobile in vitro flowering was promoted in plants cultivated in ½ MS supplemented with 30 g L-1 sucrose, 5.5 g L-1 agar, 100 mL L-1 coconut water, pH 6.0, and 2.0 mg L-1 TDZ at a controlled temperature of 18 °C and a photoperiod of 16 h. The in vitro flowering induction protocol of the D. nobile species could be used or improved for future studies.

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Author Biographies

Michele Carla Nadal, Universidad Viña del Mar

Escuela de Ciencias Agrícolas y Veterinarias, Viña del Mar, Valparaíso, Chile.

Gracielle Vidal Silva Andrade, Universidade Federal de Lavras

Escola de Ciências Agrárias, Departamento de Agricultura, Lavras-MG, Brazil.

José Henrique Nunes Flores, Universidad Viña del Mar

Escuela de Ciencias Agrícolas y Veterinarias, Viña del Mar, Valparaíso, Chile.

Michele Valquíria dos Reis, Universidade Federal de Lavras

Escola de Ciências Agrárias, Departamento de Agricultura, Lavras-MG, Brazil.

Vantuil Antonio Rodrigues, Universidade Federal de Lavras

Escola de Ciências Agrárias, Departamento de Agricultura, Lavras-MG, Brazil.

Moacir Pasqual, Universidade Federal de Lavras

Escola de Ciências Agrárias, Departamento de Agricultura, Lavras-MG, Brazil.

References

BOTELHO, F.B.S.; RODRIGUES, C.S.; BRUZI, A.T. Ornamental plant breeding. Ornamental Horticulture, v.21, n.1, p.9-16, 2015. https://doi.org/10.14295/rbho.v21i1.770

BRIDGEN, M.P.; VAN HOUTVEN, W.; EECKHAUT, T. Plant tissue culture techniques for breeding. In: VAN HUYLENBROECK, J. (eds). Ornamental Crops. Handbook of Plant Breeding, Cham: Springer, 2018. p.127-144.

CAMPOS, K.O.; KERBAUY, G.B. Thermoperiodic effect on flowering and endogenous hormonal status in Dendrobium (Orchidaceae). Journal of Plant Physiology, v.161, n.12, p.1385-1387, 2004. https://doi.org/10.1016/j.jplph.2004.07.008

CHANDLER, J. W. The Hormonal regulation of flower development. Journal of Plant Growth Regulation, v. 30, n. 2, p. 242–254, 2011.

DINANI, E.T.; SHUKLA, M.R.; TURI, C.E.; SULLIVAN, J.A.; SAXENA, P.K. Thidiazuron: modulator of morphogenesis in vitro. In: AHMAD, N.; FAISAL, M. (eds). Thidiazuron: From Urea Derivative to Plant Growth Regulator. Singapore: Springer, 2018. p.1-36.

ENG, W.-H.; HO, W.-S. Polyploidization using colchicine in horticultural plants: A review. Scientia Horticulturae, v.246, p.604–617, 2019. https://doi.org/10.1016/j.scienta.2018.11.010

HUYLENBROECK, J. VAN; BHATTARAI, K. Ornamental plant breeding: entering a new era? Ornamental Horticulture, v.28, n.3, p.297-305, 2022. https://doi.org/10.1590/2447-536x.v28i3.2516

KAUR, S. In vitro florigenesis with special reference to Orchids - A review. Recent Patents on Biotechnology, v.16, n.4, p.311-318, 2022. https://doi.org/10.2174/1872208316666220415124439

KNUDSON, L. A new nutrient solution for the germination of orchid seed. American Orquid Society Bulletim, v.14, p.214-217, 1946.

LI, S.; ZHOU, J.; XU, S.; LI, J.; LIU, J.; LU, Y.; SHI, J.; ZHOU, S.; WU, Q. Induction of Nrf2 pathway by Dendrobium nobile Lindl. alkaloids protect against carbon tetrachloride induced acute liver injury. Biomedicine and Pharmacotherapy, v.117, p.109073, 2019. https://doi.org/10.1016/j.biopha.2019.109073

MOHAMMADI, M.; KAVIANI, B.; SEDAGHATHOOR, S. In vivo polyploidy induction of Phalaenopsis amabilis in a bubble bioreactor system using colchicine. Ornamental Horticulture, v.27, n.2, p.204-212, 2021. https://doi.org/10.1590/2447-536x.v27i2.2275

MURASHIGE, T., SKOOG, F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, v.15, p.473-497, 1962. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

NOHALES, M.A.; KAY, S.A. Gigantea gates gibberellin signaling through stabilization of the DELLA proteins in Arabidopsis. Proceedings of the National Academy of Sciences, v.116, n.43, p.21893-21899, 2019. https://doi.org/10.1073/pnas.1913532116

PUJARI, I.; SANKAR BABU, V. Precocious in vitro flowering in threatened ornamental orchid, Dendrobium ovatum – Decoding the causal factors. Current Plant Biology, v.31, p.100257, 2022. https://doi.org/10.1016/j.cpb.2022.100257

REFLORA. (2020). Herbário Virtual. Available at: <https://reflora.jbrj.gov.br/reflora/herbarioVirtual/> Accessed on: January 30th 2023.

SARSAIYA, S.; JAIN, A.; JIA, Q.; FAN, X.; SHU, F.; CHEN, Z.; ZHOU, Q.; SHI, J.; CHEN, J. Molecular identification of endophytic fungi and their pathogenicity evaluation against Dendrobium nobile and Dendrobium officinale. International Journal of Molecular Sciences, v.21, n.1, p.1-16, 2020. https://doi.org/10.3390/ijms21010316

SILVA, J.A.T.; KERBAUY, G.B.; ZENG, S.; CHEN, Z.; DUAN, J. In vitro flowering of orchids. Critical Reviews in Biotechnology, v.34, n.1, p.56-76, 2014. https://doi.org/10.3109/07388551.2013.807219

SILVA, J.A.T.D.A; NG, T.B. The medicinal and pharmaceutical importance of Dendrobium species. Applied Microbiology and Biotechnology, v.101, n.6, p.2227-2239, 2017. https://doi.org/10.1007/s00253-017-8169-9

TAIZ, L.; ZEIGER, E.; MOLLER, I. M.; MURPHY, A. Plant Physiology and Development. 6ed. Sunderland: Sinauer Associates, Inc., Publishers. 2017. 888p.

WANG, S.-L.; AN, H. R.; TONG, C.-G.; JANG, S. Flowering and flowering genes: from model plants to orchids. Horticulture, Environment, and Biotechnology, v.62, n.2, p.135-148, 2021. https://doi.org/10.1007/s13580-020-00309-8

WANG, S.-L.; VISWANATH, K.K.; TONG, C.-G.; AN, H.R.; JANG, S.; CHEN, F.-C. Floral induction and flower development of orchids. Frontiers in Plant Science, v.10, p. 1-15, 2019. https://doi.org/10.3389/fpls.2019.01258

WANG, Z.H.; WANG, L.; YE, Q.S. High frequency early flowering from in vitro seedlings of Dendrobium nobile. Scientia Horticulturae, v.122, n.2, p.328-331, 2009. https://doi.org/10.1016/j.scienta.2009.05.018

WEN, Z.; GUO, W.; LI, J.; LIN, H.; HE, C.; LIU, Y.; ZHANG, Q.; LIU, W. Comparative transcriptomic analysis of vernalization- and cytokinin-induced floral transition in Dendrobium nobile. Scientific Reports, v.7, p.45748, 2017. https://doi.org/10.1038/srep45748

ZHANG, D.; LIAO, Y.; LU, S.; LI, C.; SHEN, Z.; YANG, G.; YIN, J. Effect of thidiazuron on morphological and flowering characteristics of Dendrobium ‘Sunya Sunshine’ potted plants. New Zealand Journal of Crop and Horticultural Science, v.47, n.3, p.170-181, 2019. https://doi.org/10.1080/01140671.2019.1576744

ZHAO, D.; HU, G.; CHEN, Z.; SHI, Y.; ZHENG, L.; TANG, A.; LONG, C. Micropropagation and in vitro flowering of Dendrobium wangliangii: A critically endangered medicinal orchid. Journal of Medicinal Plants Research, v.7, n.28, p.2098-2110, 2013. https://doi.org/10.5897/JMPR11.1777

ZHENG, J.; MA, Y.; ZHANG, M.; LYU, M.; YUAN, Y.; WU, B. Expression pattern of FT/TFL1 and miR156- Targeted SPL genes associated with developmental stages in Dendrobium catenatum. International Journal of Molecular Sciences, v.20, n.11, p.2725, 2019. https://doi.org/10.3390/ijms20112725

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Published

2023-05-22

Issue

Vol. 29 No. 2 (2023)

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