Indução de florescimento in vitro de Dendrobium nobile
DOI:
https://doi.org/10.1590/2447-536X.v29i2.2612Palavras-chave:
florescimento, temperatura, thidiazuron, OrchidaceaeResumo
A floração in vitro é uma técnica utilizada no melhoramento genético que acelerar gerações e favorecer a fixação mais rápida de novos caracteres de interesse agronômico e do mercado. O estudo visou estabelecer um protocolo para a floração in vitro de Den- drobium nobile, através dos efeitos combinados de temperatura e do regulador de crescimento thidiazuron (TDZ). Os experimentos foram realizados em incubadora tipo BOD a 18, 21, 24 °C e, em sala de crescimento a 26 °C. As concentrações de TDZ testadas foram de 0,0, 0,5, 1,0 e 2,0 mg L-1. A maior porcentagem de brotos floridos de 13,70% ocorreu na concentração de 2 mg L-1 de TDZ cultivados em temperatura de 18 °C. O florescimento in vitro de D. nobile foi promovido em plantas cultivadas em ½ MS suplementadas com 30 g L-1 de sacarose, 5,5 g L-1 de ágar, 100 mL L-1 de água de coco, pH 6,0 e 2,0 mg L-1 de TDZ em tempera- tura controlada de 18 °C e fotoperíodo de 16 h. O protocolo de indução de florescimento in vitro da espécie D. nobile poderá ser utilizado ou aprimorado para estudos futuros.
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Copyright (c) 2023 Michele Carla Nadal, Gracielle Vidal Silva Andrade, José Henrique Nunes Flores, Michele Valquíria dos Reis, Vantuil Antonio Rodrigues, Moacir Pasqual
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
